For the cultivation and enumeration of fecal coliforms in water by the membrane-filtration technique at a high temperature
Applications
- Detection / Cultivation / Enumeration
- COLIFORMS IN WATER
Packaging
Suspend 37.1 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Cool to 45-50ºC and aseptically add 2 vials, each vial for 500 ml of the medium, of Fecal Coliforms Supplement (Cat. 6023), previously reconstituted in 5 ml of 1% 0.2 N NaOH solution. Boil for one minute until complete dissolution. Homogenize gently and dispense into Petri dishes. DO NOT AUTOCLAVE.
Fecal Coliforms Supplement (Cat. 6023).
Rosolic Acid ..................... 50 mg
Formula
- Lactose 12.5
- Tryptose 10
- Proteose Peptone N°3 5
- Sodium Chloride 5
- Yeast Extract 3
- Bile Salts N°3 1.5
- Aniline Blue 0.1
