For the cultivation of Bacteroides fragilis and for phage recovery from environmental samples
Applications
- Cultivation
- BACTEROIDES FRAGILIS
Packaging
Suspend 29.42 grams of the medium in one liter of distilled water. Add 1ml of Calcium Chloride Dihydrate CaCl2 X H2O 5%. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50ºC. Aseptically add 1ml/litre of 1% hemin sterile solution prepared in 0.02% NaOH. Mix well. Just before use add 25 ml/litre of a sterile solution of 10.6% (w/v) of Disodium Carbonate. Adjust the pH to 7.0 with HCl.
Formula
- Peptone 10
- Tryptone 10
- Sodium Chloride 5
- Yeast Extract 2
- Glucose 1.8
- L-Cysteine 0.5
- Magnesium Sulfate Heptahydrate 0.12
