D-2 Agarose has a higher gelling temperature than D-1 Agarose. This gives higher thermal stability to the gels.
Packaging
500 g
D-2 Agarose has a higher gelling temperature than D-1 Agarose and Low EEO*.
This gives higher thermal stability to the gels.
Features
- Extraordinary mechanical resistance for more reliable and easier handling.
- Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
- Excellent transparency of the gels.
- Excellent elasticity and flexibility of the gels.
- Great capacity for derivatization and cross-linking, which allows coupling of enzymes, antigens and other substances to the gel structure.
- Exceptionally low absorption of staining agents.
- Absence of toxicity (the alternative is polyacrylamide which can be toxic).
Applications
D-2:
- Nucleic acid electrophoresis.
- Protein electrophoresis (immunoelectrophoresis and counterelectrophoresis).
- Preparation of agarose beads.
Specifications and Functional Tests D-2
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Moisture ≤ 8%
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Ash ≤ 0.4%
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EEO* ≤ 0.14 Sulfate ≤ 0.2%
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Clarity 1.5% (NTU) ≤ 4
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Gel Strength 1% (g/cm2) ≥ 900
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Gel Strength 1.5% (g/cm2) ≥1200
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Gelling Temperature 1.5% (°C) 42±1.5
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Melting Temperature 1.5% (°C) 87±1.5
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DNAse/RNAse activity None detected
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DNA resolution ≥1000bp Finely resolved
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Gel background Very low
*EEO (electroendosmosis)