D-2 Agarose has a higher gelling temperature than D-1 Agarose. This gives higher thermal stability to the gels.
Packaging
100 g
D-2 Agarose has a higher gelling temperature than D-1 Agarose and Low EEO*.
This gives higher thermal stability to the gels.
Features
- Extraordinary mechanical resistance for more reliable and easier handling.
- Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
- Excellent transparency of the gels.
- Excellent elasticity and flexibility of the gels.
- Great capacity for derivatization and cross-linking, which allows coupling of enzymes, antigens and other substances to the gel structure.
- Exceptionally low absorption of staining agents.
- Absence of toxicity (the alternative is polyacrylamide which can be toxic).
Applications
D-2:
- Nucleic acid electrophoresis.
- Protein electrophoresis (immunoelectrophoresis and counterelectrophoresis).
- Preparation of agarose beads.
Specifications and Functional Tests D-2
-
Moisture ≤ 8%
-
Ash ≤ 0.4%
-
EEO* ≤ 0.14 Sulfate ≤ 0.2%
-
Clarity 1.5% (NTU) ≤ 4
-
Gel Strength 1% (g/cm2) ≥ 900
-
Gel Strength 1.5% (g/cm2) ≥1200
-
Gelling Temperature 1.5% (°C) 42±1.5
-
Melting Temperature 1.5% (°C) 87±1.5
-
DNAse/RNAse activity None detected
-
DNA resolution ≥1000bp Finely resolved
-
Gel background Very low
*EEO (electroendosmosis)