Packaging

500 g

D-5 Agarose is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic, added to the very low sulfate content, produces an strong intercatenary interaction, yielding a gel with very high gel strength and higher exclusion limit.

Features:

- Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes, but also with large sized particles like viruses and ribosomes.

- High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used. 

- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.

- Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).

- Exceptionally low absorption of staining agents.

- Absence of toxicity (the alternative is polyacrylamide which can be toxic).

Applications:

- Conventional Electrophoresis: can be used in a wide range of concentrations.

- Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.

- Blotting.

- Agarose Beads preparation.

- Cell and enzyme immobilization.

Specifications and Functional

• Tests D-5 Moisture ≤ 7%
• Ash ≤ 0.25%
• EEO* ≤ 0.12
• Sulfate ≤ 0.12%
• Clarity 1.5%
• (NTU) ≤ 4
• Gel Strength 1% (g/cm2) ≥ 1800
• Gel Strength 1.5% (g/cm2) ≥ 3200
• Gelling Temperature 1.5% (°C) 36±1.5
• Melting Temperature 1.5% (°C) 88±1.5
• DNAse/RNAse activity None detected
• DNA resolution ≥1000 bp Finely resolved
• Gel background Very low

*EEO (electroendosmosis)