Selective medium for detection and enumeration of Listeria monocytogenes

Applications

• Detection / Enumeration
• Selective enrichment
• LISTERIA
• CHROMOGENIC MEDIA

Packaging

Suspend 35.275 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. To prepare more quantity of 500 ml, it is recommended to sterilize at 115 ° C for 10 minutes. Cool to 45-50ºC and aseptically add one vial of Listeria Lipase C Supplement (Cat. 6031) and one vial of Listeria Chromogenic Selective Supplement (Cat. 6040), previously reconstituted in 5 ml of sterile distilled water/acetone 1:1. Homogenize gently and dispense into Petri dishes.

LISTERIA LIPASE C Supp.(Cat.6031)(1 vial for 500 ml of the medium)Lipase C Substrate 10 ml

LISTERIA CHROMOGENIC SELECTIVE SUPPLEMENT(6040)(1 vial for 500 ml of the medium)Cycloheximide .25 mg,Ceftazidime .10 mg,Nalidixic acid .10 mg,Polymyxin B . 38350 IU

Formula

• Enzymatic Digest of Animal Tissue: 18
• Lithium Chloride 10
• Yeast Extract 10
• Enzymatic Digest of Casein 6
• Sodium Chloride 5
• Disodium Hydrogen Phosphate 2.5
• Glucose 2
• Sodium pyruvate 2
• Magnesium Glycerophospate 1
• Magnesium sulphate 0.5
• 5 Bromo- 4 chloro-3-indolyl β-D-glucopyranoside 0.05
• Bacteriological Agar 13.5