For the isolation and enumeration of heterotrophic marine bacteria
Applications
- Isolation / Enumeration
- MARINE HETEROTROPHIC BACTERIA
Packaging
Suspend 55.2 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes.Cool to 50ºC, mix well and dispense into plates. The colour is amber, slightly opalescent,and may present a light precipitation. It is recommended to homogenize the medium in its container before pouring into plates.
Formula
- Sodium Chloride 19.4
- Magnesium Chloride 8.8
- Bacteriological Peptone 5
- Sodium Sulfate 3.24
- Calcium Chloride 1.8
- Yeast Extract 1
- Potassium Chloride 0.55
- Sodium Bicarbonate 0.16
- Ferric Citrate 0.1
- Potassium Bromide 0.08
- Strontium Chloride 0.034
- Boric Acid 0.022
- Disodium Phosphate 0.008
- Sodium Silicate 0.004
- Sodium Fluoride 0.0024
- Ammonium Nitrate 0.0016
- Bacteriological Agar 15
