For the cultivation and enumeration of Clostridium spp and other anaerobes
Applications
- Clostridium
- ISOLATION AND IDENTIFICATION
Packaging
Suspend 50 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50ºC and, if desired, add 0.02 g/l of Polymyxin B in a sterile filtered solution.
Formula
- Beef Extract 10
- Peptone 10
- Dextrose 5
- Sodium Chloride 5
- Yeast Extract 3
- Sodium Acetate 3
- Soluble Starch 1
- L-Cysteine Hydrochloride 0.5
- Bacteriological Agar 12.5
