Differential medium for studies of Gram negative, non fermenting bacteria
Applications
- Identification / Differentiation
- NON FERMENTING BACILLI
Packaging
Suspend 43.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121ºC for 10 minutes. Allow to cool in a slanted position in order to obtain butts of 3.5 cm depth and a slant length of 7-7.5 cm.
Important: Immediately before inoculation, add 0.15 ml or 2 drops of 50% dextrose aqueous solution, allowing it to run down the side of the tube opposite to the slant.
Formula
- Gelatin Peptone 20
- D-Mannitol 2
- Sodium Chloride 2
- Magnesium Sulfate 1.5
- Dipotassium Phosphate 1
- L-Arginine 1
- Sodium Nitrate 1
- Yeast Extract 1
- Sodium Nitrite 0.35
- Bromothymol Blue 0.04
- Phenol Red 0.008
- Bacteriological Agar 13.5
