For the detection and enumeration of E.coli and coliforms in drinking water by membrane filtration technique
Applications
- Detection / Cultivation / Enumeration
- COLIFORMS IN WATER
Packaging
Suspend 56.15 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 3 ml of TTC 1% Supplement (Cat. 6030) previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes. DO NOT OVERHEAT THE MEDIUM.
TTC 1% SUPPLEMENT (CAT. 6030)
1 Vial reconstructed in 5 ml of distilled water
Triphenyltetrazolium Chloride …………………………… 50 mg
Formula
- Lactose 20
- Peptone 10
- Yeast Extract 6
- Beef Extract 5
- Sodium Heptadecyl Sulfate (Tergitol 7) 0.1
- Bromothymol Blue 0.05
- Bacteriological Agar 15
