Selective medium for the detection and enumeration of coliforms in dairy products, water and food
Applications
- Detection / Enumeration
- COLIFORMS
Packaging
Suspend 41.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT AUTOCLAVE. Cool to 45ºC and use immediately. If desired, it can also be dispensed and sterilized in the autoclave at 118ºC for 15 minutes, as Hartham demonstrated that media prepared only by boiling gave the same results as media autoclaved.
Formula
- Lactose 10
- Gelatin Peptone 7
- Sodium Chloride 5
- Yeast Extract 3
- Bile Salts Nº3 1.5
- Neutral Red 0.03
- Crystal Violet 0.002
- Bacteriological Agar 15
