For the detection and enumeration of Enterococcus in water through the single step membrane filtration technique
Applications
- Detection / Enumeration
- ENTEROCOCCUS IN WATER
- CHROMOGENIC MEDIA
Packaging
Suspend 71.95 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 50ºC, mix well and dispense into plates. For a more selective medium, prepare a solution of 0.24 grams of nalidixic acid in 5 ml of sterile distilled water with a few drops of sodium hydroxide 0.1N (for a better dissolution), and aseptically add to one liter of medium. If desired, 15 ml per liter of a 1% TTC solution can be added.
Formula
- Yeast Extract 30
- Sodium Chloride 15
- Peptone 10
- Esculine 1
- X-Glucoside 0.75
- Sodium Azide 0.15
- Cycloheximide 0.05
- Bacteriological Agar 15
