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codice: NDRND2000C
NanoDrop 2000c UV-Vis Spectrophotometer w. cuvette

Enhance the limit of detection for DNA, RNA and protein with the 
Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer featuring both microvolume pedestal and cuvette measurement options.  
The cuvette station is ideal for measuring dilute nucleic acid and protein assays, includes heating and microstirring capabilities, and allows users to perform kinetics and cell culture readings (600nm).

NanoDrop 2000c Spectrophotometer features:

  • Wide spectral range (190-840nm) for measuring a variety of samples types:
    • Peptides (205nm)
    • DNA and RNA (260nm)
    • Purified protein (280nm)
    • Toxicology assays and industrial dyes (490nm)
    • Gold nanoparticles (520nm)
    • Colorimetric protein assays (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
    • Optical Density measurements (600nm)
  • Combining the patented sample retention system with cuvette capability accommodates low and high concentrations 
    (0.4 to 15,000 ng/µL dsDNA and 0.01 – 400 mg/mL BSA)
  • Pedestal measurements that require only 0.5 – 2 µL of your sample and do not require sample dilution, even for highly concentrated samples
  • Calculates sample purity ratios (260/280 nm and 260/230 nm)
  • Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
  • Fast measurement time of less than 5 seconds, 10 -15 seconds from sample pipette to wipe
  • User-friendly software that includes custom methods and data export capabilities

Specifications


Instrument Type   Spectrophotometer  
Minimum Sample Size 0.5 µl
Sample Number 1
Path Length 1 mm (auto-ranging to 0.05 mm)
Light Source(s) Xenon flash lamp
Detector Type 2048 - element linear silicon CCD array
Wavelength Range 190 - 840 nm
Wavelength Accuracy 1 nm
Spectral Resolution ≤ 1.8 nm (FWHM at Hg 253.7 nm)
Absorbance Precision 0.002 (1 mm path)
Absorbance Accuracy 3% (at 0.74 at 350 nm)
Absorbance Range 0.04 - 300 (10 mm equivalent)
Detection Limit 2 ng/µl (dsDNA)
Maximum Concentration 15,000 ng/µl (dsDNA)
Measurement Time < 5 seconds
Footprint 14 x 20 cm
Weight 2.1 kg
Sample Pedestal Material of Construction 303 stainless steel and quartz fiber
Operating Voltage 12 vdc
Operating Power Consumption 12 - 18 W (max 30 W)
Standby Power Consumption  5 W
Software Compatibility Operating software for the NanoDrop 2000 and NanoDrop 2000c. Compatible with Professional versions of Windows 8.1 (64 bit), and Windows 7 (32 and 64 bit).
   
Cuvette specifications  
   
Z-Height 8.5 mm
Heating 37 ± 0.5 °C
Stirrer: 150-850 rpm
Path Length 10, 5, 2, 1 mm
Absorbance Range 0.008 - 1.5
Detection Limit 0.4 ng/µl (dsDNA)
Maximum Concentration 750 ng/µl (dsDNA)
Measurement Time < 3 seconds
Cuvette Dimensions 12.5 mm x 12.5 mm, up to 48 mm H
Type Masked cuvette
UL/CSA and CE All units are approved to these standards

Software



Classic NanoDrop Applications

  • Nucleic Acid
  • Protein A280
  • Micro Array 
  • Proteins & Labels
  • BCA, Bradford, Lowry, 
    Pierce 660 nm
  • UV-Vis
  • Cell Cultures
  • plus Diagnostics

New Applications

  • User Defined custom methods
  • Kinetics

New and updated

  • Intuitive user interface
  • Flexible reporting & data export
  • Oligo calculator, units flexibility & more

FAQS 


Q: How is the Thermo Scientific NanoDrop 2000 different from the NanoDrop™ 1000? 
A: The NanoDrop 2000 UV-Vis spectrophotometer is the next generation instrument of the NanoDrop 1000. It can do everything that the full-spectrum UV-Vis NanoDrop 1000 spectrophotometer does, plus more, including: faster measurements (< 5 secs), smaller sample size (0.5 µl for higher concentration samples), broader concentration dynamic range (up to 15,000 ng/µl dsDNA), lower wavelength capability (e.g. 205 nm), and enhanced software that allows for custom methods.

Q: What is the difference between the NanoDrop 2000 and the NanoDrop 2000c? 
A: Both models have the micro-volume pedestal capabilities described above. In addition, the NanoDrop 2000c has a built-in cuvette capability which allows for all the same pedestal applications plus kinetics (time and time/temp) applications.

Q: What are some of the pre-configured applications? 
A: Nucleic acid, MicroArray, Protein A280, Proteins & Labels, Colorimetric protein methods (BCA, Bradford, Lowry, Pierce 660 nm), UV-Vis and Cell Cultures.  Same as the NanoDrop 1000 with the addition of custom methods capabilities.

Q: Is there an ability to create and save custom methods. 
A: Yes. Methods can be built (with or without standard curves), with customized analysis wavelengths, factors, extinction coefficients and more.

Q: Can kinetics be performed with these instruments? 
A: Yes, the NanoDrop 2000c model can perform kinetics using the cuvette capability. The kinetics application allows for time-based measurements as well as temperature control (37 °C).

Q: What sort of accuracy should I expect with the NanoDrop 2000/2000c? 
A: Typically within 2%.

Q: What sort of reproducibility should I expect with the NanoDrop 2000/2000c? 
A: Typically ± 2 ng/ul for dsDNA samples <= 100 ng/ul and ± 2% for samples > 100 ng/µl.

Q: Do nucleic acids require purification prior to measurement on the NanoDrop 1000? 
A:  Yes, absorbance measurements are not specific for a particular nucleic acid and will be affected by the presence of nucleotides and other molecules which absorb at 260 nm.

Q: Do proteins require purification prior to measurement on the NanoDrop 1000? 
A: Yes when using the Protein A280 module since absorbance measurements will be affected by the presence of non-protein molecules which absorb at 280 nm. For non-purified proteins there are other pre-configured modules (BCA, Bradford, Lowry, Pierce 660 nm) that may be used.

Q: Is simply wiping the pedestal surfaces enough to prevent carryover? 
A: Yes. The highly polished quartz and stainless steel surfaces of the sample retention system are resistant to sample adherence, making the use of dry lab wipes very effective in removing the sample.

Q: Can I check whether my instrument is calibrated and running accurately? 
A: Yes. We recommend performing a Calibration Check using CF-1 calibration check fluid every 6 months. CF-1 is available from NanoDrop Products or an authorized distributor.

Q: Does the NanoDrop 2000/2000c require a computer to operate? 
A: Yes, these instruments connect via USB to any PC-compatible computer running Windows 7, Windows XP or Vista.

Q: What warranties* are available? 
A: A one year warranty is included with the purchase of an instrument. In addition 1-3 year extended service plans are available. Each service plan includes parts, labor and Priority Overnight shipment to and from our service facility. All service plans include one preventative maintenance service per year. (*Applies to US/Canada only. All others please contact your local sales channel for country specific warranty and service options.)



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