For differentiation of Enterobacteriaceae on the basis of citrate utilization
Applications
- Diferenciación
- Identification / Differentiation
- Differentiatium
- COLIFORMS
- ENTEROBACTERIA
- YERSINIA
Packaging
Suspend 24.3 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121ºC for 15 minutes. Allow to cool in a slanted position in order to obtain short butts of 1- 1.5 cm. depth. Alternatively, the medium can be poured into Petri dishes.
Formula
- Sodium Chloride 5
- Sodium Citrate 2
- Ammonium Dihydrogen Phosphate 1
- Dipotassium Phosphate 1
- Magnesium Sulfate 0.2
- Bromothymol Blue 0.08
- Bacteriological Agar 15
