For the cultivation of Brucella in diverse clinical specimens, foods and other materials of sanitary interest.
Applications
Packaging
Suspend 43.1 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 5% sterile sheep defibrinated blood. Homogenize gently and dispense into Petri dishes. Once solidified, invert the plates to dry up any excess of moisture. Brucella Agar can be made selective to yield higher numbers of positive isolations by aseptically adding two vials of Brucella Supplement (Cat. 6017) previously reconstituted in 10 ml of 1:1 solution of methanol / sterile distilled water. If desired, the reconstituted Polyenrichment Supplement (Cat. 6011) can be added aseptically.
Formula
- Meat Peptone 10
- Casein Peptone 10
- Sodium Chloride 5
- Yeast Extract 2
- Dextrose 1
- Sodium Bisulfite 0.1
- Bacteriological Agar 15
