Recommended for the isolation of
Gram-positive cocci of clinical samples and other materials when used with blood
Applications
- Cultive / Isolation
- Isolation
- STAPHYLOCOCCUS
- ESTREPTOCOCCUS
Packaging
Suspend 44 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. AVOID OVERHEATING. Cool to 45-50°C and aseptically add 5-10% sterile defibrinated sheep blood, homogenize gently and pour into Petri dishes.
Formula
- Peptone Mixture 20
- Sodium Chloride 5
- Beef Extract 3
- Corn Starch 1
- Nalidixic Acid 0.015
- Colistin Sulfate 0.01
- Bacteriological Agar 15
