E AGAROSE
E AGAROSE (1 Kg)
Cat nº 8100
ideal for routine rapid separations of DNA and RNA fragments as well as PCR products, the preparation of plasmids, and for screening, cloning and blotting techniques.
Packaging
1 Kg
Agarose E is ideal for routine rapid separations of DNA and RNA fragments as well as PCR products, the preparation of plasmids, and for screening, cloning and blotting techniques.
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Easy dissolution and rapid gelling
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Excellent transparency and low background staining gives clear band visibility
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Sharp and well defined bands
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Very low DNA binding
Agarose E has high gel strength even at low concentrations, so use rates are 0.75 - 2%. It is effective in blotting and in separations of nucleic acid fractions from 250 bp to 23 Kb.
Technical Specifications
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Ash Content ≤ 0.45%
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Sulfate ≤ 0.1%
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Clarity 1.5 % (NTU) ≤ 4 NTU
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Gel Strength 1% (g/cm2) ≥1000
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Gel Strength 1.5 % (g/cm2) ≥ 2200
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Gelling Temperature 1.5 % (°C) 36 ± 1.5
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Melting Temperature 1.5 % (°C) 88 ± 1.5
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DNAse/ RNAse activity None detected
Applications
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High electrophoresis mobility
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Nucleic acid analytical and preparative electrophoresis
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Blotting assays
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Protein electrophoresis such as radial immunodiffusion
