For the differentiation of Gram negative Enterobacteria
Applications
- Diferenciación
- COLIFORMS
- ENTEROBACTERIA
Packaging
Suspend 52 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121ºC for 15 minutes. Allow to cool in a slanted position in order to obtain butts of 1.5-2 cm depth. For best results, Kliger Iron Agar should be used on the day of preparation or melted and solidified before use.
Formula
- Peptone Mixture 20
- Lactose 10
- Sodium Chloride 5
- Dextrose 1
- Ferric Ammonium Citrate 0.5
- Sodium Thiosulfate 0.5
- Phenol Red 0.025
- Bacteriological Agar 15
