Selective medium for the cultivation of Legionella
Applications
Packaging
Suspend 2.5 grams of the medium in 90 ml of distilled water. Heat until boiling and until the medium is completely dissolved. Distribute into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. Cool to 45 - 50°C and aseptically add 1 vial of Legionella CYE Growth Supplement (Cat. 6022), previously reconstituted in 10 ml of sterile distilled water and, if desired, also add 1 ml of 1 vial of LEGIONELLA GVPC SUPPLEMENT (Cat. 6025), previously reconstituted with 5 ml of sterile 1:1 water/acetone (see Preparation of Cat. 6025). Mix well and distribute into appropriate containers
Legionella CYE Growth Supplement (Cat. 6022): For 100 ml of base medium: Potassium Hydroxide (200 mg), L-Cysteine (40 mg), Ferric Pyrophosphate (25 mg), Buffer ACES (1.0 g), Alpha Ketoglutarate (0.1 g)
Legionella GVPC Supplement (Cat. 6025): For 500 ml of base medium: Polymyxin (39600 IU), Glycine (1.5 g), Cycloheximide (40 mg), Vancomycin (0.5 mg)
Caution: Legionella GVPC Supplement contains cycloheximide and is very toxic if swallowed, inhaled or comes into contact with the skin. Wear gloves and eye/face protection.
Formula
- Yeast Extract 10
- Activated Charcoal 2
- Bacteriological Agar 13
