Selective and differential medium for the detection of Listeria spp, particularly Listeria monocytogenes
Applications
Packaging
Suspend 34.4 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Palcam Listeria Selective Supplement (Cat. 6004),previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes.
Formula
- Peptones 23
- Lithium Chloride 15
- Mannitol 10
- Yeast Extract 3
- Esculin 0.8
- Glucose 0.5
- Ferric Ammonium Citrate 0.5
- Phenol Red 0.08
- Sodium Chloride 5
- Maize Starch 1
- Bacteriological Agar 10
