Enrichment medium for the detection and isolation of Listeria in food and environmental samples
Applications
- Selective enrichment
- LISTERIA
Packaging
Suspend 28.7 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Ferric Ammonium Citrate Supplement (Cat. 6050), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into sterile containers.
Formula
- Sodium Chloride 20
- Disodium Phosphate 12
- Tryptone 5
- Meat Peptone 5
- Beef Extract 5
- Yeast Extract 5
- Lithium Chloride 3
- Monopotassium Phosphate 1.35
- Esculin 1
- Acriflavine 0.025
- Nalidixic Acid 0.02
