Selective medium for the cultivation of Lactobacilli in medical and food microbiology
Applications
- Cultivation
- LACTIC ACID BACTERIA
Packaging
Suspend 75 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Add 1.32 ml of Acetic Acid Glacial and mix well. Heat again at 90-100°C for two minutes. DO NOT AUTOCLAVE. Cool the medium to 40–45°C and dispense into sterilized appropriate containers.
Formula
- Sodium Acetate 15
- Tryptone 10
- Dextrose 10
- Monopotassium Phosphate 6
- Yeast Extract 5
- Sucrose 5
- Arabinose 5
- Ammonium Citrate 2
- Polysorbate 80 1
- Magnesium Sulfate 0.57
- Manganese Sulfate 0.12
- Ferrous Sulfate 0.03
- Bacteriological Agar 15
