In combination with MUP, allows the direct detection of viable Bifidobacteria
Applications
Packaging
Suspend 62.5 grams of the medium in 950 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. This medium is heat sensitive. It is recommended to autoclave small quantities (95-190 ml). Sterilize in autoclave at 115±3ºC for 15 minutes. Cool to 48ºC in a water bath and if desired, aseptically add two vials of MUP Selective Supplement (Cat. 6074) reconstituted in 5 ml of sterile distilled water.
NOTE: After autoclaving the medium is slightly opalescent. The opalescency disappear when the medium is cooled to 48±1ºC. The base is stable during 4 hours in a water bath.
Formula
- Sodium Propionate 15
- Casein Peptone 10
- Galatooligosaccharide TOS 10
- Dipotassium Phosphate 4.8
- Monopotassium Phosphate 3
- Ammonium Sulfate 3
- Yeast Extract 1
- L-Cysteine HCl 0.5
- Magnesium Sulfate Heptahydrated 0.2
- Bacteriological Agar 15
