codice: 1533
THIOGLYCOLLATE MEDIUM USP

THIOGLYCOLLATE MEDIUM USP ISO 7937

For the sensitivity test according to American Pharmacopoeia, and for the cultivation of Clostridium perfringens according ISO 7937

Applications
  • Sterility Test Media
  • Cultivation
  • Isolation CLOSTRIDIUM PERFRINGENS
  • OTHER APPLICATIONS
Packaging

Suspend 30 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. Cool to room temperature (25ºC). If the stored medium exhibits more than 20% pink color (due to oxidation), the tubes should be reheated in a water bath to expel the oxygen. Do not reheat more than once.

Formula
  • Enzymatic Digest of Casein 15
  • Dextrose 5.5
  • Yeast Extract 5
  • Sodium Chloride 2.5
  • Sodium Thioglycollate 0.5
  • L-Cystine 0.5
  • Resazurin 0.001
  • Bacteriological Agar 0.75


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